It really depends where your water source and drain vents can be located.
That's a decent sized room! Bigger than both of my bathrooms put together. Layout is crucial. You need to know how to run plumbing, gas, electrical and sewer lines. You need a contractor for bids.
Get at least 3 bids if not more from licensed contractors and see if it fits within your budget. Building codes need to be followed, permits pulled and inspections performed.
Not a small project, and if you can do it, certainly would add value to the home if done properly.
Before you do anything, check your local building codes. You may need a permit to install your water lines and drains. It also depends on what you want in your room. Do you want both a bath and a laundry style room? Or just one or the other? Which ever you want, get some graph paper (or find a place online where you can do a mock room), measure the room exactly then the size of each item you want in the room. Arrange them and rearrange them until you are satisfied with the potential look. Then you'll need a plumber to go over your plan to see if the water lines and drains will work in your home. I hope this helps you start your research into your project. Wishing you the best.
We turned a closet into a bathroom - with no rough in- here’s how: https://www.frugalfamilytimes.com/2017/11/no-rough-in-no-problem-why-we-chose-saniflo-for-basement-bathroom.html?m=1
If there is no rough-in already for a toilet stack, construction and installation of one will likely be one of the largest expenses. Drilling down into the lower foundation of the house to attach the new plumbing lines with the existing sewer lines will be required. Although it is possible, the cost could easily be well into the 10's of thousands.