How to remove mold from a glass picture frame?
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You did not mention if this is a painting, but here are instructions for removing mold from a painting:
https://www.thoughtco.com/removing-mold-from-a-painting-2578829
You will need to replace the matting.
I’d ask a pro. Mold is nothing to fool around with.
It looks like it might be on the inside - open up the picture and clean the glass, maybe it was stored in a damp environment
I would try taking it apart and cleaning it. even if you mess it up, at least you tried.
A little bleach in water should do the trick on the glass If you can take the picture out of the frame and lay it in the open air and maybe some sun--it will help. However--not so much sun that you fade the picture.
I would just replace the glass